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Anti-SOX11 Antibody

Western blot analysis of SOX11 expression in Hela (A), DLD (B) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46; 70 kD)
Immunofluorescent analysis of SOX11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P35716
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46679 Da

Additional Information
Gene ID	6664
Other Names	Transcription factor SOX-11
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SOX11. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SOX11
Function	Transcription factor that acts as a transcriptional activator (PubMed:24886874, PubMed:26543203). Binds cooperatively with POU3F2/BRN2 or POU3F1/OCT6 to gene promoters, which enhances transcriptional activation (By similarity). Acts as a transcriptional activator of TEAD2 by binding to its gene promoter and first intron (By similarity). Plays a redundant role with SOX4 and SOX12 in cell survival of developing tissues such as the neural tube, branchial arches and somites, thereby contributing to organogenesis (By similarity).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00267, ECO:0000269\|PubMed:24886874, ECO:0000269\|PubMed:35938035}
Tissue Location	Expressed primarily in the brain and heart, with low expression in the kidney, pancreas and muscle

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Jay P.,et al.Genomics 29:541-545(1995).
Azuma T.,et al.DNA Res. 6:357-360(1999).
Hillier L.W.,et al.Nature 434:724-731(2005).
Goze C.,et al.Nucleic Acids Res. 21:2943-2943(1993).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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¥ 1,250.00

Cat# AP52016

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Anti-SOX11 Antibody

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