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Anti-TRPM8 Antibody

Western blot analysis of TRPM8 expression in mouse lung (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 130 kD)
Immunohistochemical analysis of TRPM8 staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TRPM8 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q7Z2W7
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	127685 Da

Additional Information
Gene ID	79054
Other Names	LTRPC6; TRPP8; Transient receptor potential cation channel subfamily M member 8; Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRPM8. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TRPM8
Synonyms	LTRPC6, TRPP8
Function	Non-selective ion channel permeable to monovalent and divalent cations, including Na(+), K(+), and Ca(2+), with higher permeability for Ca(2+). Activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. Activated by cool temperatures (<23-28 degrees Celsius) and by chemical ligands evoking a sensation of coolness, such as menthol and icilin therefore plays a central role in the detection of environmental cold temperatures (PubMed:15306801, PubMed:15852009, PubMed:16174775, PubMed:25559186, PubMed:37857704). TRPM8 is a voltage-dependent channel; its activation by cold or chemical ligands shifts its voltage thresholds towards physiological membrane potentials, leading to the opening of the channel (PubMed:15306801). In addition to its critical role in temperature sensing, regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality (By similarity). May play a role in prostate cancer cell migration (PubMed:16174775, PubMed:25559186).
Cellular Location	Cell membrane; Multi-pass membrane protein. Membrane raft {ECO:0000250\|UniProtKB:Q8R4D5}. Endoplasmic reticulum membrane. Note=Lipid raft association modulates TRPM8 channel activity (By similarity) Located in the endoplasmic reticulum in prostate cancer cells (PubMed:11325849, PubMed:16174775). {ECO:0000250\|UniProtKB:Q8R4D5, ECO:0000269\|PubMed:11325849, ECO:0000269\|PubMed:16174775}
Tissue Location	Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Tsavaler L.,et al.Cancer Res. 61:3760-3769(2001).
Thebault S.,et al.J. Biol. Chem. 280:39423-39435(2005).
Bidaux G.,et al.J. Biol. Chem. 287:2948-2962(2012).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Sano Y.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP52018

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Anti-TRPM8 Antibody

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