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Thyroid hormone receptor alpha Rabbit pAb

Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Thyroid hormone receptor alpha antibody(AP52112), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Thyroid hormone receptor alpha (AP52112) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P10827
Reactivity	Rat, Human, Mouse
Predicted	Pig, Rabbit, Chicken, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	54816 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Thyroid hormone receptor alpha
Epitope Specificity	81-180/490
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus.
DISEASE	Defects in THRA are the cause of congenital hypothyroidism non-goitrous type 6 (CHNG6) [MIM:614450]. A disease characterized by growth retardation, developmental retardation, skeletal dysplasia, borderline low thyroxine levels and high triiodothyronine levels. There is differential sensitivity to thyroid hormone action, with retention of hormone responsiveness in the hypothalamic pituitary axis and liver but skeletal, gastrointestinal, and myocardial resistance.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

Additional Information
Gene ID	7067
Other Names	Thyroid hormone receptor alpha, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, EAR-7, c-erbA-1, c-erbA-alpha, THRA, EAR7, ERBA1, NR1A1, THRA1, THRA2
Dilution	WB=1:500-2000,Flow-Cyt=1ug/Test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	THRA
Synonyms	EAR7, ERBA1, NR1A1, THRA1, THRA2
Function	[Isoform Alpha-1]: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Cellular Location	Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Laudet V.,et al.Nucleic Acids Res. 19:1105-1112(1991).
Miyajima N.,et al.Cell 57:31-39(1989).
Nakai A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:2781-2785(1988).
Pfahl M.,et al.Nucleic Acids Res. 15:9613-9613(1987).
Nakai A.,et al.Mol. Endocrinol. 2:1087-1092(1988).

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¥ 1,250.00

Cat# AP52112

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

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Availability: 3-5 days

询价

Thyroid hormone receptor alpha Rabbit pAb