> 首页 > 产品 > 一抗 > 精选抗体 > G蛋白偶联受体抗体（GPCR） > DRD1 Rabbit pAb

DRD1 Rabbit pAb

Blank control: Hela(blue). Primary Antibody:Rabbit Anti- Dopamine Receptor D1 antibody(AP52160), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (AP52160, 1ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of AP52160 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DRD1 Polyclonal Antibody, Unconjugated(AP52160) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	P21728
Reactivity	Rat, Human
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	49293 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human DRD1
Epitope Specificity	101-200/446
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Additional Information
Gene ID	1812
Other Names	D(1A) dopamine receptor, Dopamine D1 receptor, DRD1
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1 µg/Test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DRD1
Function	Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Forms heterotetramers with DRD3 to potentiate beta-arrestin recruitment and mediate locomotor activity.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P18901}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P18901}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P18901}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P18901}. Cell projection, cilium membrane; Multi-pass membrane protein. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q61616}. Cell projection, dendritic spine {ECO:0000250\|UniProtKB:Q61616}. Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 {ECO:0000250\|UniProtKB:P18901}
Tissue Location	Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Dearry A.,et al.Nature 347:72-76(1990).
Zhou Q.-Y.,et al.Nature 347:76-80(1990).
Sunahara R.K.,et al.Nature 347:80-83(1990).
Ohara K.,et al.Neuropsychopharmacology 8:131-135(1993).
Suwa M.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP52160

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

reduce add

Availability: 3-5 days

询价

DRD1 Rabbit pAb

DRD1 Rabbit pAb

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务