IL12A Rabbit pAb

Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-IL12 (AP52181) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(AP52181) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(AP52181-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	P29459
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	24874 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human IL-12
Epitope Specificity	51-150/219
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	bs-0767P is one synthetic peptide derived from mouse IL-12. IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Additional Information
Gene ID	3592
Other Names	Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, NKSF1, IL12A, NKSF1
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IL12A
Synonyms	NKSF1
Function	Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed:8605935, PubMed:8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed:1673147, PubMed:1674604, PubMed:8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed:8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed:7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed:7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed:22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed:22306691).
Cellular Location	Secreted

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Wolf S.F.,et al.J. Immunol. 146:3074-3081(1991).
Gubler U.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147(1991).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Stern A.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:6808-6812(1990).
Merberg D.M.,et al.Immunol. Today 13:77-78(1992).

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¥ 1,250.00

Cat# AP52181

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

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Availability: 3-5 days

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IL12A Rabbit pAb

IL12A Rabbit pAb

BACKGROUND

REFERENCES

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