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phospho-mu Opioid Receptor (Ser375) Rabbit pAb
phospho-mu Opioid Receptor (Ser375) Rabbit pAb
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Application 
| WB |
|---|---|
| Primary Accession | P33535 |
| Predicted | Rat, Pig, Dog, Human, Mouse, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 44494 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from rat mu Opioid Receptor around the phosphorylation site of Ser375 |
| Epitope Specificity | HP(p-S)TA |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
| Gene ID | 25601 |
|---|---|
| Other Names | Mu-type opioid receptor, M-OR-1, MOR-1, Opioid receptor B, Oprm1, Ror-b |
| Dilution | WB=1:500-2000,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Oprm1 |
|---|---|
| Synonyms | Ror-b |
| Function | Receptor for endogenous opioids such as beta-endorphin and endomorphin (PubMed:11060299, PubMed:15944153, PubMed:16682964, PubMed:17384143, PubMed:17947509, PubMed:1846076, PubMed:18558479, PubMed:21292762, PubMed:7595566, PubMed:7678862, PubMed:8051154, PubMed:8240812, PubMed:8393525, PubMed:9224819, PubMed:9572309). Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone (PubMed:11060299, PubMed:15944153, PubMed:16682964, PubMed:17384143, PubMed:17947509, PubMed:1846076, PubMed:18558479, PubMed:21292762, PubMed:7595566, PubMed:7678862, PubMed:8051154, PubMed:8240812, PubMed:8393525, PubMed:9224819, PubMed:9572309). Also activated by enkephalin peptides, such as Met-enkephalin or Met-enkephalin-Arg-Phe, with higher affinity for Met-enkephalin-Arg-Phe (PubMed:8624732). Agonist binding to the receptor induces coupling to an inactive GDP- bound heterotrimeric G protein complex and subsequent exchange of GDP for GTP in the G protein alpha subunit leading to dissociation of the G protein complex with the free GTP-bound G protein alpha and the G protein beta-gamma dimer activating downstream cellular effectors (PubMed:16682964, PubMed:9224819). The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15 (PubMed:9224819, PubMed:9572309). They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen- activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B (PubMed:15944153, PubMed:21292762, PubMed:7595566, PubMed:9572309). Also couples to adenylate cyclase stimulatory G alpha proteins (PubMed:7595566). The selective temporal coupling to G proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization (PubMed:11060299, PubMed:17384143, PubMed:17947509, PubMed:18558479). Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G protein-dependent or a beta- arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation (PubMed:11278523, PubMed:11896051, PubMed:15944153). Acts as a class A G protein-coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G protein coupling (PubMed:11060299, PubMed:17384143, PubMed:17947509, PubMed:18558479). Endogenous ligands induce rapid desensitization, endocytosis and recycling. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties (PubMed:16682964, PubMed:17384143). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P42866}. Cell projection, axon {ECO:0000250|UniProtKB:P97266}. Perikaryon {ECO:0000250|UniProtKB:P97266}. Cell projection, dendrite {ECO:0000250|UniProtKB:P97266}. Endosome {ECO:0000250|UniProtKB:P97266}. Note=Is rapidly internalized after agonist binding. {ECO:0000250|UniProtKB:P97266} |
| Tissue Location | Brain. Is expressed in the cerebral cortex, caudate putamen, nucleus accumbens, septal nuclei, thalamus, hippocampus, and habenula. Not detected in cerebellum |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
REFERENCES
Fukuda K.,et al.FEBS Lett. 327:311-314(1993).
Wang J.-B.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10230-10234(1993).
Chen Y.,et al.Mol. Pharmacol. 44:8-12(1993).
Bunzow J.R.,et al.Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
Thompson R.C.,et al.Neuron 11:903-913(1993).
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