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CMKLR1 Rabbit pAb

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Primary: Anti-CMKLR1 (AP52238) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(AP52238) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse
Predicted	Rat, Dog, Sheep
Host	Rabbit
Clonality	Polyclonal
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from rat CMKLR1
Epitope Specificity	151-250/371
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane; Multi-pass membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Chemokine-Like Receptor 1 (CMKLR1) is a serpentine transmembrane G-protein linked receptor. It is an orphan chemoattractant receptor, also known as ChemR23 or DEZ. CMKLR1 is expressed on the surface of monocyte-derived immature dendritic cells and peripheral plamacytoid dendritic (pDC) cells; but not on myeloid dendritic (mDC) cells, monocytes and lymphocytes. The expression of CMKLR1 on pDC is down-regulated after activation. Chemerin (TIG2) is a natural ligand of CMKLR1. The interaction of CMKLR1 with Chemerin induces pDC activation and chemotaxis. It has been shown that CMKLR1 is a coreceptor for several strains of SIV and HIV-1. CMKLR1 is a potential useful marker for distinguishing pDCs and mDCs.

Additional Information
Other Names	DEZ; Gpcr27; ChemR23; mcmklr1; Chemokine-like receptor 1; G-protein coupled receptor DEZ; Cmklr1
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Methner A.,et al.Biochem. Biophys. Res. Commun. 233:336-342(1997).
Arita M.,et al.J. Exp. Med. 201:713-722(2005).
Zabel B.A.,et al.Exp. Hematol. 34:1106-1114(2006).
Roh S.G.,et al.Biochem. Biophys. Res. Commun. 362:1013-1018(2007).
Goralski K.B.,et al.J. Biol. Chem. 282:28175-28188(2007).

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¥ 1,250.00

Cat# AP52238

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

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Availability: 3-5 days

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CMKLR1 Rabbit pAb