Nrf2 Rabbit pAb

The Hela（treated with MG-132 (10 µM,10h) ） (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Nrf2 antibody (AP52269): 1 µg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (AP52269-BF488): 1 µg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela（treated with MG-132 (10 µM,10h) ）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Nrf2) polyclonal Antibody, unconjugated (AP52269) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, AP52269-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Hela (H) cells were treated with or without MG-132 (10 µM) for 10h, 25 µg total protein per lane of cell lysates (see on figure) probed with Nrf2 monoclonal antibody, unconjugated (bsm-61093R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (AP52269) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q16236
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	68 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Nrf2
Epitope Specificity	401-500/605
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
SIMILARITY	Belongs to the bZIP family. CNC subfamily.Contains 1 bZIP domain.
SUBUNIT	Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1.
Post-translational modifications	Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors.

Additional Information
Other Names	Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nrf-2, Nuclear factor, erythroid derived 2, like 2, NFE2L2 {ECO:0000303\|PubMed:29018201, ECO:0000312\|HGNC:HGNC:7782}
Target/Specificity	Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Dilution	WB=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,Flow-Cyt=1 µg/Test,ICC/IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription
factor which regulates the expression of many detoxification and
antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors.

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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¥ 1,500.00

Cat# AP52269

Size:

50 µl

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

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Availability: 3-5 days

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Nrf2 Rabbit pAb

Nrf2 Rabbit pAb

BACKGROUND

REFERENCES

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