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SYNPR/Synaptoporin Rabbit pAb

Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(AP52298), Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Sample:
Bulbus olfactorius (Rat) Lysate at 40 ug
Primary: Anti-Synaptoporin (AP52298) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (AP52298) at 1:800 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q8TBG9
Reactivity	Rat
Predicted	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29166 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Synaptoporin
Epitope Specificity	31-130/265
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Synaptic vesicle recycling involves numerous proteins that contribute to the formation and trafficking of the SNARE complexes throughout the cell. Synaptoporin, also designated synaptophysin 2, is an integral membrane protein of small synaptic vesicles that belongs to the synaptophysin/synaptobrevin family. Synaptoporin is highly homologus to Synaptophysin 1 and both Synaptoporin and synaptophysin 1 contain four transmembrane domains and a short cytoplasmic tail. The Synaptoporin protein also contains one MARVEL domain, a membrane-associating domain found in lipid-associating proteins, and displays calcium-binding activity which may be localized to its cytoplasmic tail. Syntaphilin, synaptophysin and Synaptoporin regulate the formation of the vesicles by competing with components of the SNARE complexes to respectively inhibit either the assembly or the secretion of the synaptic vesicles.

Additional Information
Gene ID	132204
Other Names	Synaptoporin, SYNPR
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1 µg/Test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SYNPR
Function	Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein (By similarity).
Cellular Location	Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Synapse, synaptosome

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Mao Y.,et al.Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP52298

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

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Availability: 3-5 days

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SYNPR/Synaptoporin Rabbit pAb