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IL-17B Rabbit pAb

IL-17B Rabbit pAb

     
  • 14 - IL-17B Rabbit pAb AP52336
    Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-17B) Polyclonal Antibody, Unconjugated (AP52336) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF
Primary Accession Q9QXT6
Reactivity Rat, Human
Predicted Pig, Dog, Mouse, Horse
Host Rabbit
Clonality Polyclonal
Calculated MW 20309 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from mouse IL-17B
Epitope Specificity 21-120/180
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Secreted.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. [provided by RefSeq, Jul 2008]
Additional Information
Gene ID 56069
Other Names Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name Il17b
Synonyms Nirf, Zcyto7
Function Stimulates the release of tumor necrosis factor alpha and IL- 1-beta from the monocytic cell line THP-1.
Cellular Location Secreted.
Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Presnell S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Moore E.E.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Zhang W.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).

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