UNC50 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-UNC50 Antibody (N-term) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
UNC50 Antibody (N-term) (Cat. #AP5294a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, WB, E
Primary Accession	Q53HI1
Other Accession	O55227, Q9CQ61, Q7ZUU1, Q3ZBG6
Reactivity	Human
Predicted	Bovine, Zebrafish, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	30373 Da
Antigen Region	5-33 aa

Additional Information
Gene ID	25972
Other Names	Protein unc-50 homolog, Periodontal ligament-specific protein 22, PDLs22, Protein GMH1 homolog, hGMH1, Uncoordinated-like protein, UNC50, UNCL
Target/Specificity	This UNC50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human UNC50.
Dilution	FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	UNC50 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	UNC50
Synonyms	UNCL
Function	Involved in the cell surface expression of neuronal nicotinic receptors (By similarity). Binds RNA (By similarity).
Cellular Location	Nucleus inner membrane {ECO:0000250\|UniProtKB:O55227}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:O55227}. Golgi apparatus membrane; Multi-pass membrane protein
Tissue Location	Present in periodontal ligament fibroblasts (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.