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Anti-MRRF Antibody

Western blot analysis of MRRF expression in COS7 (A), A549 (B), MCF7 (C), CT26 (D), PC12 (E) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 26 kD)
Immunohistochemical analysis of MRRF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q96E11
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29277 Da

Additional Information
Gene ID	92399
Other Names	Ribosome-recycling factor mitochondrial; RRF; Ribosome-releasing factor mitochondrial
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MRRF. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MRRF (HGNC:7234)
Function	Responsible for the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis (PubMed:19716793, PubMed:33878294). Acts in collaboration with GFM2 (PubMed:33878294). Promotes mitochondrial ribosome recycling by dissolution of intersubunit contacts (PubMed:33878294).
Cellular Location	Mitochondrion.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Zhang Y.,et al.Biochim. Biophys. Acta 1443:245-250(1998).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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¥ 1,250.00

Cat# AP53308

Size:

50 µl

100 µl

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¥ 1,250.00

¥ 2,050.00

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Anti-MRRF Antibody

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