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Anti-CNTROB Antibody

Western blot analysis of CNTROB expression in HepG2 (A), HUVEC (B) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 100 kD)
Immunohistochemical analysis of CNTROB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8N137
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	101253 Da

Additional Information
Gene ID	116840
Other Names	LIP8; Centrobin; Centrosomal BRCA2-interacting protein; LYST-interacting protein 8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CNTROB. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CNTROB
Synonyms	LIP8
Function	Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Centriole-associated, asymmetrically localizes to the daughter centriole
Tissue Location	Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Zou C.,et al.J. Cell Biol. 171:437-445(2005).
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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¥ 1,250.00

Cat# AP53335

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100 µl

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Anti-CNTROB Antibody

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