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Anti-Collagen 19 alpha 1 Antibody

Western blot analysis of Collagen 19 alpha 1 expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 115 kD)
Immunohistochemical analysis of Collagen 19 alpha 1 staining in human thymus gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen 19 alpha 1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q14993
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	115221 Da

Additional Information
Gene ID	1310
Other Names	Collagen alpha-1(XIX) chain; Collagen alpha-1(Y) chain
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 19 alpha 1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	COL19A1
Function	May act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular matrix or the sphinteric smooth muscle.
Cellular Location	Secreted, extracellular space, extracellular matrix
Tissue Location	Localized to vascular, neuronal, mesenchymal, and some epithelial basement membrane zones in umbilical cord

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Inoguchi K.,et al.J. Biochem. 117:137-146(1995).
Khaleduzzaman M.,et al.Genomics 45:304-312(1997).
Mungall A.J.,et al.Nature 425:805-811(2003).
Yoshioka H.,et al.Genomics 13:884-886(1992).
Myers J.C.,et al.Gene 123:211-217(1993).

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¥ 1,250.00

Cat# AP53336

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Anti-Collagen 19 alpha 1 Antibody

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