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Anti-MRPS9 Antibody

Western blot analysis of MRPS9 expression in HEK293T (A), Hela (B), mouse kidney (C) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 46 kD)
Immunohistochemical analysis of MRPS9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P82933
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	45835 Da

Additional Information
Gene ID	64965
Other Names	RPMS9; 28S ribosomal protein S9 mitochondrial; MRP-S9; S9mt
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPS9. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MRPS9
Synonyms	RPMS9
Cellular Location	Mitochondrion.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Koc E.C.,et al.J. Biol. Chem. 276:19363-19374(2001).
Choudhary C.,et al.Science 325:834-840(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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¥ 1,250.00

Cat# AP53355

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Anti-MRPS9 Antibody

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