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Human IgG (9G6) Mouse mAb

     
  • 1 - Human IgG (9G6) Mouse mAb AP53502
    Western blot detection of human IgG using human IgG mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Reactivity Human
Host Mouse
Clonality Monoclonal Antibody
Isotype IgG1
Conjugate Unconjugated
Immunogen Human IgG was isolated from human sera and purified by chromatography.
Purification Affinity Purified
Calculated MW 55 KDa
Additional Information
Other Names N/A
Dilution WB~~1:1000
Format Liquid in Purified antibody in PBS with 0.05% sodium azide.
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

BACKGROUND

Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

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