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Anti-Catenin alpha 1/2 Antibody

Western blot analysis of Catenin alpha 1/2 expression in MCF7 (A), A431 (B), SHSY5Y (C), PC12 (D) whole cell lysates.
Immunohistochemical analysis of Catenin alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P35221
Other Accession	P26232
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	100071 Da

Additional Information
Gene ID	1495
Other Names	CTNNA1; Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; CTNNA2; CAPR; Catenin alpha-2; Alpha N-catenin; Alpha-catenin-related protein
Target/Specificity	Recognizes endogenous levels of Catenin alpha 1/2 protein.
Dilution	WB~~1/500 - 1/1000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CTNNA1 (HGNC:2509)
Function	Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1- dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.
Cellular Location	Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:P26231}. Cell junction, adherens junction. Cell membrane {ECO:0000250\|UniProtKB:P26231}; Peripheral membrane protein; Cytoplasmic side {ECO:0000250\|UniProtKB:P26231}. Cell junction Cytoplasm {ECO:0000250\|UniProtKB:Q9PVF8}. Nucleus. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries. {ECO:0000250\|UniProtKB:P26231}
Tissue Location	[Isoform 1]: Ubiquitously expressed in normal tissues.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol