Anti-NT5C1B Antibody

Western blot analysis of NT5C1B expression in BV2 (A), MCF7 (B), H1792 (C) whole cell lysates.
Immunohistochemical analysis of NT5C1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NT5C1B staining in EAhy926 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	Q96P26
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	68804 Da

Additional Information
Gene ID	100526794;93034
Other Names	AIRP; Cytosolic 5'-nucleotidase 1B; cN1B; Autoimmune infertility-related protein; Cytosolic 5'-nucleotidase IB; cN-IB
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NT5C1B. The exact sequence is proprietary.
Dilution	WB~~1/500 - 1/1000 IF~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	NT5C1B
Synonyms	AIRP
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, AMP is the major substrate.
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q9BXI3}.
Tissue Location	Highly expressed in testis, placenta and pancreas. Detected at lower levels in heart, kidney, liver and lung

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.