Anti-Cytochrome P450 27C1 Antibody

Western blot analysis of Cytochrome P450 27C1 expression in HepG2 (A), HEK293T (B), Panc1 (C), rat kidney (D) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 42; 55 kD)
Immunofluorescent analysis of Cytochrome P450 27C1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q4G0S4
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	60920 Da

Additional Information
Gene ID	339761
Other Names	Cytochrome P450 27C1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 27C1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYP27C1 (HGNC:33480)
Function	[Isoform 2]: A cytochrome P450 monooxygenase that catalyzes the 3,4 desaturation of all-trans-retinol (also called vitamin A1) to all-trans-3,4-didehydroretinol (also called vitamin A2) in the skin. Desaturates with lower efficiency all-trans retinal and all-trans retinoic acid. Forms minor amounts of 3-hydroxy and 4-hydroxy all- trans-retinol derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Cellular Location	[Isoform 2]: Mitochondrion membrane {ECO:0000250\|UniProtKB:P14137}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P14137}
Tissue Location	Widely expressed, with highest levels in the liver, kidney and pancreas.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.