Anti-GGT5 HC Antibody

Western blot analysis of GGT5 HC expression in H460 (A), LO2 (B), mouse kidney (C), MCF7 (D), HuT78 (E) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)
Immunofluorescent analysis of GGT5 HC staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P36269
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	62261 Da

Additional Information
Gene ID	2687
Other Names	GGTLA1; Gamma-glutamyltransferase 5; GGT 5; Gamma-glutamyl transpeptidase-related enzyme; GGT-rel; Gamma-glutamyltransferase-like activity 1; Gamma-glutamyltranspeptidase 5; Glutathione hydrolase 5; Leukotriene-C4 hydrolase
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GGT5 HC. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GGT5
Synonyms	GGTLA1
Function	Cleaves the gamma-glutamyl bond of extracellular glutathione tripeptide (gamma-Glu-Cys-Gly) and certain glutathione conjugates (PubMed:1676842, PubMed:21447318). Hydrolyzes glutathione releasing L- Glu and Cys-Gly dipeptide which is further metabolized to maintain extracellular cysteine levels but also to provide cysteine necessary for intracellular glutathione synthesis (PubMed:1676842, PubMed:21447318). Among glutathione-S-conjugates metabolizes leukotriene C4 (LTC4) and S-geranylgeranyl-glutathione (GGG), but is inactive toward gamma-glutamyl leucine. Converts extracellular LTC4 to LTD4 during acute inflammatory response. Acts as a negative regulator of GGG bioactivity. GGT5 (via GGG catabolism) and ABCC1 (via extracellular transport) establish GGG gradients within lymphoid tissues to position P2RY8-positive lymphocytes at germinal centers in lymphoid follicles and restrict their chemotactic transmigration from blood vessels to bone marrow parenchyma (By similarity). The transpeptidation reaction, i.e. the transfer of gamma-glutamyl moiety to an acceptor molecule to yield a new gamma-glutamyl compound requires high concentration of dipeptide acceptor and is considered nonphysiological (PubMed:21447318, PubMed:29667297).
Cellular Location	Membrane {ECO:0000250\|UniProtKB:Q9Z2A9}; Single- pass type II membrane protein {ECO:0000250\|UniProtKB:Q9Z2A9}
Tissue Location	Expressed in follicular dendritic cells in lymphoid follicles (at protein level).