Anti-GPR68 Antibody

Western blot analysis of GPR68 expression in HeLa (A), mouse spleen (B) whole cell lysates.
Immunofluorescent analysis of GPR68 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	Q15743
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	OGR1; Ovarian cancer G-protein coupled receptor 1; OGR-1; G-protein coupled receptor 68; GPR12A; Sphingosylphosphorylcholine receptor
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR68. The exact sequence is proprietary.
Dilution	WB~~1/500 - 1/1000 IF~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt