Anti-MYO1D Antibody

Western blot analysis of MYO1D expression in rat brain (A), A2780 (B) whole cell lysates.
Immunohistochemical analysis of MYO1D staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	O94832
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	116202 Da

Additional Information
Gene ID	4642
Other Names	KIAA0727; Unconventional myosin-Id
Target/Specificity	Recognizes endogenous levels of MYO1D protein.
Dilution	WB~~1/500 - 1/1000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	MYO1D
Synonyms	KIAA0727
Function	Unconventional myosin that functions as actin-based motor protein with ATPase activity (By similarity). Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes (By similarity). Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q63357}. Perikaryon {ECO:0000250\|UniProtKB:Q63357}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q63357}. Early endosome {ECO:0000250\|UniProtKB:F1PRN2}. Cytoplasm, cell cortex {ECO:0000250\|UniProtKB:Q63357}. Note=Colocalizes with the actin cytoskeleton in the cell cortex close to the apical cell membrane Colocalizes with cytoplasmic puncta that are reminiscent of transport vesicles. {ECO:0000250\|UniProtKB:Q63357}
Tissue Location	Expressed in many tissues. Highest levels in brain, followed by lung and ovary; expression is lowest in spleen

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.