Anti-5-HT7 Antibody

Western blot analysis of 5-HT7 expression in Hela (A), H1688 (B) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)
Immunofluorescent analysis of 5-HT7 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P34969
Reactivity	Human, Mouse, Rat, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53555 Da

Additional Information
Gene ID	3363
Other Names	5-hydroxytryptamine receptor 7; 5-HT-7; 5-HT7; 5-HT-X; Serotonin receptor 7
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 5-HT7. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HTR7 {ECO:0000303\|Ref.3, ECO:0000312\|HGNC:HGNC:5302}
Function	G protein-coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed:35714614, PubMed:8226867). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:35714614, PubMed:8226867). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:35714614).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	[Isoform A]: Predominant isoform in spleen, caudate and hippocampus. [Isoform D]: Minor isoform in terms of expression.