Anti-VAMP1/2/3 Antibody

Western blot analysis of VAMP1/2/3 expression in HepG2 (A), mouse brain (B) whole cell lysates.
Immunohistochemical analysis of VAMP1/2/3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P23763
Other Accession	P63027, Q15836
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	12902 Da

Additional Information
Gene ID	6843
Other Names	VAMP1; SYB1; Vesicle-associated membrane protein 1; VAMP-1; Synaptobrevin-1; VAMP2; SYB2; Vesicle-associated membrane protein 2; VAMP-2; Synaptobrevin-2; VAMP3; SYB3; Vesicle-associated membrane protein 3; VAMP-3; Cellubrevin; CEB; Synaptobrevin-3
Target/Specificity	Recognizes endogenous levels of VAMP1/2/3 protein.
Dilution	WB~~1/500 - 1/1000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	VAMP1
Synonyms	SYB1
Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Cellular Location	[Isoform 1]: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Synapse, synaptosome [Isoform 3]: Mitochondrion outer membrane; Single-pass type IV membrane protein
Tissue Location	Nervous system, skeletal muscle and adipose tissue.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.