Anti-CCZ1 Antibody

Western blot analysis of CCZ1 expression in A549 (A), HEK293T (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 56 kD)
Immunofluorescent analysis of CCZ1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P86791
Other Accession	P86790
Reactivity	Human, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	55866 Da

Additional Information
Gene ID	221960;51622
Other Names	CCZ1; C7orf28A; CGI-43; Vacuolar fusion protein CCZ1 homolog; CCZ1B; C7orf28B; Vacuolar fusion protein CCZ1 homolog B; Vacuolar fusion protein CCZ1 homolog-like
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CCZ1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCZ1
Synonyms	C7orf28A
Function	Acts in concert with MON1A, as a guanine exchange factor (GEF) for RAB7, promotes the exchange of GDP to GTP, converting it from an inactive GDP-bound form into an active GTP-bound form (PubMed:23084991).
Cellular Location	Lysosome membrane.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.