Anti-GTF2H1 Antibody

Western blot analysis of GTF2H1 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 65 kD)
Immunohistochemical analysis of GTF2H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of GTF2H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P32780
Reactivity	Human, Mouse, Rat, Pig, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	62032 Da

Additional Information
Gene ID	2965
Other Names	BTF2; General transcription factor IIHC subunit 1; Basic transcription factor 2 62 kDa subunit; BTF2 p62; General transcription factor IIHC polypeptide 1; TFIIHC basal transcription factor complex p62 subunit
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GTF2H1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GTF2H1 (HGNC:4655)
Synonyms	BTF2
Function	Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Cellular Location	Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.