Anti-PKE Antibody

Western blot analysis of PKE expression in CT26 (A), PC12 (B), HEK293T (C), A549 (D), MCF7 (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 55 kD)
Immunohistochemical analysis of PKE staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of PKE staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q86UX6
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	54994 Da

Additional Information
Gene ID	282974
Other Names	YANK3; Serine/threonine-protein kinase 32C; PKE; Yet another novel kinase 3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKE. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt