Anti-SRSF5 Antibody

Western blot analysis of SRSF5 expression in HeLa (A), RAW264.7 (B), H9C2 (C) whole cell lysates.
Immunohistochemical analysis of SRSF5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SRSF5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	Q13243
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	31264 Da

Additional Information
Gene ID	6430
Other Names	HRS; SFRS5; SRP40; Serine/arginine-rich splicing factor 5; Delayed-early protein HRS; Pre-mRNA-splicing factor SRP40; Splicing factor, arginine/serine-rich 5
Target/Specificity	Recognizes endogenous levels of SRSF5 protein.
Dilution	WB~~1/500 - 1/2000 IF~~1/50 - 1/100
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	SRSF5
Synonyms	HRS, SFRS5, SRP40
Function	Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.