Anti-MYCBP Antibody

Western blot analysis of MYCBP expression in A549 (A), rat lung (B) whole cell lysates.
Immunohistochemical analysis of MYCBP staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q99417
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	11967 Da

Additional Information
Gene ID	26292
Other Names	AMY1; C-Myc-binding protein; Associate of Myc 1; AMY-1
Target/Specificity	Recognizes endogenous levels of MYCBP protein.
Dilution	WB~~1/500 - 1/2000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	MYCBP (HGNC:7554)
Synonyms	AMY1
Function	May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
Cellular Location	Cytoplasm. Nucleus. Mitochondrion. Note=Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1
Tissue Location	Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.