Anti-GPRC5C Antibody

Western blot analysis of GPRC5C expression in HEK293T (A), MCF7 (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 48 kD)
Immunofluorescent analysis of GPRC5C staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9NQ84
Reactivity	Human, Mouse, Rat, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48193 Da

Additional Information
Gene ID	55890
Other Names	RAIG3; G-protein coupled receptor family C group 5 member C; Retinoic acid-induced gene 3 protein; RAIG-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC5C. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPRC5C
Synonyms	RAIG3
Function	This retinoic acid-inducible G protein-coupled receptor provide evidence for a possible interaction between retinoid and G- protein signaling pathways.
Cellular Location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein Note=Localized in the plasma membrane and perinuclear vesicles
Tissue Location	Expression is highest in the periphery, particularly in the stomach, but also in the kidney, liver, pancreas, and prostate. In brain, levels of expression are generally lower than in the periphery, with the exception of cerebellum, spinal cord, and dorsal root ganglia (DRG).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.