Anti-RASGEF1A Antibody

Western blot analysis of RASGEF1A expression in MCF7 (A), U87MG (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)
Immunohistochemical analysis of RASGEF1A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8N9B8
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	54556 Da

Additional Information
Gene ID	221002
Other Names	Ras-GEF domain-containing family member 1A
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RASGEF1A. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RASGEF1A
Function	Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration.
Tissue Location	Detected in brain and spinal cord. Highly expressed in a number of intrahepatic cholangiocarcinoma tissue biopsies

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.