Anti-Histone H2B (Acetyl-K34) Antibody

Western blot analysis of Histone H2B (Acetyl-K34) expression in A549 (A), BV2 (B), PC12 (C) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 15 kD)
Immunofluorescent analysis of Histone H2B (Acetyl-K34) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Direct ELISA antibody dose-response curve using Anti-Histone H2B (Acetyl-K34) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P62807
Other Accession	P58876, Q93079, O60814, Q99880, Q99879, Q99877, Q5QNW6, P57053
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	13906 Da

Additional Information
Gene ID	3017;8339;8343;8344;8346;8347
Other Names	HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; HIST1H2BD; H2BFB; HIRIP2; Histone H2B type 1-D; HIRA-interacting protein 2; Histone H2B.1 B; Histone H2B.b; H2B/b; HIST1H2BH; H2BFJ; Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BK; H2BFT; HIRIP1; Histone H2B type 1-K; H2B K; HIRA-interacting protein 1; HIST1H2BL; H2BFC; Histone H2B type 1-L; Histone H2B.c; H2B/c; HIST1H2BM; H2BFE; Histone H2B type 1-M; Histone H2B.e; H2B/e; HIST1H2BN; H2BFD; Histone H2B type 1-N; Histone H2B.d; H2B/d; HIST2H2BF; Histone H2B type 2-F; H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s
Target/Specificity	KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K34 of human Histone H2B protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	H2BC4 (HGNC:4757)
Synonyms	H2BFL, HIST1H2BC
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location	Nucleus. Chromosome.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.