Anti-Histone H2A Antibody

Western blot analysis of Histone H2A expression in SHSY5Y (A), HUT78 (B), HCT116 (C) whole cell lysates. (Predicted band size: 14; 15 kD; Observed band size: 14 kD)
Immunofluorescent analysis of Histone H2A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P0C0S8
Other Accession	P16104
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	14091 Da

Additional Information
Gene ID	8329;8330;8332;8336;8969
Other Names	HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p; H2AFX; H2AX; Histone H2A.x; H2a/x
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H2A. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	H2AC11 (HGNC:4737)
Synonyms	H2AFP, HIST1H2AG
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location	Nucleus. Chromosome.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.