Anti-mPR gamma Antibody

Western blot analysis of mPR gamma expression in Hela (A), HGC27 (B), mouse kidney (C), mouse lung (D) whole cell lysates.
Immunohistochemical analysis of mPR gamma staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of mPR gamma staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	Q9NXK6
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	38014 Da

Additional Information
Gene ID	54852
Other Names	MPRG; Membrane progestin receptor gamma; mPR gamma; Progestin and adipoQ receptor family member 5; Progestin and adipoQ receptor family member V
Target/Specificity	Recognizes endogenous levels of mPR gamma protein.
Dilution	WB~~1/500 - 1/1000 IF~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	PAQR5 (HGNC:29645)
Synonyms	MPRG
Function	Plasma membrane progesterone (P4) receptor coupled to G proteins (PubMed:23763432). Seems to act through a G(i) mediated pathway (PubMed:23763432). May be involved in oocyte maturation (PubMed:12601167).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Expressed in the brain, lung, kidney, colon, adrenal and lung.