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Anti-Actin Muscle Specific Antibody

Immunohistochemical analysis of Actin Muscle Specific staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of Actin Muscle Specific staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of Actin Muscle Specific staining in human fibroid formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of Actin Muscle Specific staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Primary Accession	P68032
Other Accession	P68133, P63267
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	42019 Da

Additional Information
Gene ID	70
Other Names	ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTA3; ACTL3; ACTSG; Actin gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
Target/Specificity	Recognizes endogenous levels of Actin Muscle Specific protein.
Format	Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ACTC1
Synonyms	ACTC
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol