癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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MID2 Rabbit pAb
MID2 Rabbit pAb
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- 实验流程
- 背景知识
Application 
| IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q9UJV3 |
| Predicted | Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 83210 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Midline-2 |
| Epitope Specificity | 251-350/735 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger. |
| SUBUNIT | Homodimer or heterodimer with MID1. Interacts with IGBP1. |
| Post-translational modifications | Phosphorylated on serine and threonine residues. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine. |
| Gene ID | 11043 |
|---|---|
| Other Names | Probable E3 ubiquitin-protein ligase MID2, 2.3.2.27, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, RING-type E3 ubiquitin transferase MID2, Tripartite motif-containing protein 1, MID2, FXY2, RNF60, TRIM1 |
| Target/Specificity | Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine. |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | MID2 |
|---|---|
| Synonyms | FXY2, RNF60, TRIM1 |
| Function | E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29 (PubMed:35266954). |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton Note=Microtubule-associated. |
| Tissue Location | Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.
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