癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
IPO7 Antibody (N-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O95373 |
| Other Accession | Q9EPL8, NP_006382.1 |
| Reactivity | Human, Mouse |
| Predicted | Rat, Bovine, Canine, Rabbit, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 119517 Da |
| Antigen Region | 142-169 aa |
| Gene ID | 10527 |
|---|---|
| Other Names | Importin-7, Imp7, Ran-binding protein 7, RanBP7, IPO7, RANBP7 |
| Target/Specificity | This IPO7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-169 amino acids from the N-terminal region of human IPO7. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IPO7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | IPO7 |
|---|---|
| Synonyms | RANBP7 |
| Function | Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed:11682607). In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. Promotes odontoblast differentiation via promoting nuclear translocation of DLX3, KLF4, SMAD2, thereby facilitating the transcription of target genes that play a role in odontoblast differentiation (By similarity). Facilitates BMP4-induced translocation of SMAD1 to the nucleus and recruitment to the MSX1 gene promoter, thereby promotes the expression of the odontogenic regulator MSX1 in dental mesenchymal cells (By similarity). Also promotes odontoblast differentiation by facilitating the nuclear translocation of HDAC6 and subsequent repression of RUNX2 expression (By similarity). Inhibits osteoblast differentiation by inhibiting nuclear translocation of RUNX2 and therefore inhibition of RUNX2 target gene transcription (By similarity). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9EPL8}. Nucleus {ECO:0000250|UniProtKB:Q9EPL8}. Note=Localizes to the nucleus in the presence of BMP4. {ECO:0000250|UniProtKB:Q9EPL8} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.
REFERENCES
Huang, S., et al. Biosci. Rep. 30(3):159-168(2010)
Chachami, G., et al. Biochem. Biophys. Res. Commun. 390(2):235-240(2009)
Adeyemo, A., et al. PLoS Genet. 5 (7), E1000564 (2009) :
Zaitseva, L., et al. Retrovirology 6, 11 (2009) :
Yao, X., et al. J. Biol. Chem. 283(33):22867-22874(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Dean, K.A., et al. J. Cell. Sci. 114 (PT 19), 3479-3485 (2001) :
Jakel, S., et al. EMBO J. 18(9):2411-2423(1999)
Paraskeva, E., et al. J. Cell Biol. 145(2):255-264(1999)
Jakel, S., et al. EMBO J. 17(15):4491-4502(1998)
Gorlich, D., et al. J. Cell Biol. 138(1):65-80(1997)
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