> 首页 > 产品 > 一抗 > 其他 > C1QL1 Polyclonal Antibody

C1QL1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: mouse cerebel tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C1QL1 Polyclonal Antibody, Unconjugated(AP54354) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C1QL1) Polyclonal Antibody, Unconjugated (AP54354) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-C1QL1 (AP54354) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	O75973
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	26453 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human C1QL1
Epitope Specificity	21-120/258
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
SIMILARITY	Contains 1 C1q domain. Contains 1 collagen-like domain.
SUBUNIT	Interacts with BAI3 (By similarity).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	C1q is part of the C1 enzyme complex, which activates the serum complement system. The residues of the globular domain in C1q share homology with several other secreted and membrane-bound collagen or collagen-like proteins, including pre-cerebellin and collagen types VIII and X, as well as the human and mouse genes encoding Apm1/BPB80 and AdipoQ/ACRP30, respectively. These various C1q-related proteins are found in adipose serum, corneal endothelium, chondrocytes and cerebral Purkinje cells. C1qL1 (complement component 1, q subcomponent-like 1), also known as CRF or C1QRF, is a polypeptide with a hydrophobic signal sequence, a collagenous region and a globular domain at the carboxy terminus, which shares homology to the C1q globular domain. C1qL1 transcripts are most abundant in areas of the nervous system that are associated with motor function, including cerebral Purkinje cells, the pons, the accessory olivary nucleus, and the red nucleus. The similarity of mouse C1qL1 to human C1qL1 suggests a conserved and important role for the protein. In humans, the gene encoding C1qL1 maps to chromosome 17q21.

Additional Information
Gene ID	10882
Other Names	C1q-related factor, C1q and tumor necrosis factor-related protein 14, C1q/TNF-related protein 14, Complement component 1 Q subcomponent-like 1, C1QL1, C1QRF, CRF, CTRP14
Target/Specificity	Expressed in brainstem.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	C1QL1
Synonyms	C1QRF, CRF, CTRP14
Function	May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses (By similarity).
Cellular Location	Secreted.
Tissue Location	Expressed in brainstem.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol