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细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NPSR1 Rabbit pAb
NPSR1 Rabbit pAb
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q6W5P4 |
| Predicted | Rat, Pig, Dog, Human, Mouse, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 42687 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human NPSR1 |
| Epitope Specificity | 201-300/371 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cell Membrane and Cytoplasmic |
| DISEASE | Defects in NPSR1 are a cause of asthma-related traits type 2 (ASRT2) [MIM:608584]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy and atopic dermatitis. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR154 (G-protein coupled receptor 154), also known as NPSR1 (neuropeptide S receptor), GPRA (G-protein coupled receptor for asthma susceptibility) or PGR14, is a 371 amino acid protein that is thought to play a role in autocrine or paracrine signaling pathways. Ubiquitously expressed, GPR154 exists as nine alternatively spliced isoforms. Defects in the gene encoding GPR154 is the cause of asthma-related traits type 2 (ASRT2). |
| Gene ID | 387129 |
|---|---|
| Other Names | Neuropeptide S receptor {ECO:0000303|Ref.3}, G-protein coupled receptor 154, G-protein coupled receptor PGR14, G-protein coupled receptor for asthma susceptibility, NPSR1 |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NPSR1 |
|---|---|
| Function | G protein-coupled receptor for neuropeptide S (NPS) (PubMed:15947423, PubMed:16720571, PubMed:16790440). Receptor activation by NPS initiates a G(q)/GNAQ-dependent phospholipase C- activating signaling pathway, resulting in Ca(2+) mobilization from intracellular stores and increased intracellular Ca(2+) levels (PubMed:15312648, PubMed:15947423, PubMed:16720571, PubMed:16790440, PubMed:25714705, PubMed:26865629). In addition to this pathway, NPS binding to its receptor activates cAMP/PKA signal transduction (PubMed:26865629). Finally, both pathways converge to activate ERK1/ERK2 phosphorylation and signaling cascade (PubMed:26865629). Inhibits cell growth in response to NPS binding (PubMed:15947423). |
| Cellular Location | [Isoform 1]: Cell membrane; Multi-pass membrane protein [Isoform 4]: Cell membrane; Multi-pass membrane protein [Isoform 5]: Cytoplasm [Isoform 7]: Cytoplasm |
| Tissue Location | Isoform 4 is ubiquitous; it is detected in glandular epithelia of bronchus, stomach, small intestine, colon, uterus, esophagus, spleen, kidney, pancreas, prostate and breast Isoform 1 is detected in uterus, colon and prostate, and in the smooth muscle cell layer in bronchial and arterial walls (at protein level) (PubMed:15947423). Isoform 1 is predominantly expressed in smooth muscle. Isoform 4 is predominantly expressed in epithelial cells. In bronchial biopsies, it is expressed in smooth muscle cells of asthma patients, but not in control patients; whereas in epithelial cells, its expression is consistently stronger in asthma patients |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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