NELL2 Rabbit pAb

Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Placenta tissue lysates
Primary: Anti-NELL2 (AP54664) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 135 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL2) Polyclonal Antibody, Unconjugated (AP54664) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q99435
Reactivity	Rat, Mouse
Predicted	Pig, Dog, Human, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	91346 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human NELL2/NRP2
Epitope Specificity	201-300/816
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]

Additional Information
Gene ID	4753
Other Names	Protein kinase C-binding protein NELL2, NEL-like protein 2, Nel-related protein 2, NELL2 (HGNC:7751), NRP2
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NELL2 (HGNC:7751)
Synonyms	NRP2
Function	Plays multiple roles in neural tissues, regulates neuronal proliferation, survival, differentiation, polarization, as well as axon guidance and synaptic functions. Plays an important role in axon development during neuronal differentiation through the MAPK intracellular signaling pathway (By similarity). Via binding to its receptor ROBO3, plays a role in axon guidance, functioning as a repulsive axon guidance cue that contributes to commissural axon guidance to the midline (PubMed:32198364). Required for neuron survival through the modulation of MAPK signaling pathways too. Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).
Cellular Location	Secreted {ECO:0000250\|UniProtKB:Q62918}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.