SPOCK2 Rabbit pAb

Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-SPOCK2 (AP54690) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (AP54690) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (AP54690) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q92563
Reactivity	Rat, Human, Mouse
Predicted	Chicken, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46779 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SPOCK2/Testican 2
Epitope Specificity	61-160/424
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Additional Information
Gene ID	9806
Other Names	Testican-2, SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2, SPOCK2, KIAA0275, TICN2
Dilution	WB=1:500-2000,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SPOCK2
Synonyms	KIAA0275, TICN2
Function	May participate in diverse steps of neurogenesis. Binds calcium.
Cellular Location	Secreted, extracellular space, extracellular matrix
Tissue Location	Highly expressed in brain. Also found in lung and testis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.