DLGAP2 Rabbit pAb

Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-DLGAP2 (AP54743) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 115 kDa
Tissue/cell: Rat brain tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DLGAP2 Polyclonal Antibody, Unconjugated(AP54743) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9P1A6
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	117620 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SAPAP2/DLGAP2
Epitope Specificity	251-360/1054
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Postsynaptic density of neuronal cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	A guanylate kinase is a phosphotransferase that produces ADP and GDP from the substrates ATP and GMP. SAPAP2, also known as DAP-2 (Disks large-associated protein 2) and PSD-95/SAP90-binding protein 2, is a 1054 amino acid protein that localizes to the postsynaptic membrane of neuronal cells of the brain and kidney. SAPAP2 likely acts as a signaling molecule which interacts with the human genes DLG1 and DLG4/PSD-95. The gene encoding SAPAP2, DLGAP2, maps to human chromosome 8. Consisting of nearly 146 million base pairs, chromosome 8 encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that maps to chromosome 8.

Additional Information
Other Names	Disks large-associated protein 2, DAP-2, PSD-95/SAP90-binding protein 2, SAP90/PSD-95-associated protein 2, SAPAP2, DLGAP2 (HGNC:2906)
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DLGAP2 (HGNC:2906)
Function	May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD- 95/SAP90 at the plasma membrane.
Cellular Location	Cell membrane; Peripheral membrane protein. Postsynaptic density. Synapse. Note=Postsynaptic density of neuronal cells
Tissue Location	Expressed in brain and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol