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TXLNB Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-TXLNB (AP54751) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXLNB) Polyclonal Antibody, Unconjugated (AP54751) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q8N3L3
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	76519 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human TXLNB
Epitope Specificity	331-430/684
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the taxilin family.
SUBUNIT	Binds to the C-terminal coiled coil region of syntaxin family members STX1A, STX3A and STX4A. Has a preference for STX1A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Beta-taxilin promotes nerve regeneration and may be involved in intracellular vesicle transport. Expressed predominantly in cardiac and skeletal muscle, beta-taxilin binds to the coiled coil region of the syntaxin family members STX1A, STX3A, and STX4A. Beta- and gamma-taxilins, bind to the alpha subunit of the nascent polypeptide-associated complex (NAC) and affect its nuclear distribution, suggesting that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the transcriptional process through its interaction with alphaNAC alone.

Additional Information
Gene ID	167838
Other Names	Beta-taxilin, Muscle-derived protein 77, hMDP77, TXLNB, C6orf198, MDP77
Target/Specificity	Expressed in skeletal muscle.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	TXLNB
Synonyms	C6orf198, MDP77
Function	Promotes motor nerve regeneration (By similarity). May be involved in intracellular vesicle traffic.
Tissue Location	Expressed in skeletal muscle.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol