ANO9 Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, E
Primary Accession	A1A5B4
Predicted	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	90333 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human TP53I5/ANO9
Epitope Specificity	551-650/782
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane; Multi-pass membrane protein (Potential).
SIMILARITY	Belongs to the anoctamin family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	May act as a calcium-activated chloride channel.

Additional Information
Gene ID	338440
Other Names	Anoctamin-9, Transmembrane protein 16J, Tumor protein p53-inducible protein 5, p53-induced gene 5 protein, ANO9, PIG5, TMEM16J, TP53I5
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	ANO9
Synonyms	PIG5, TMEM16J, TP53I5
Function	PKA-activated nonselective cation channel (PubMed:29604966). Discriminates poorly among cations but is more permeable to Ca(2+) ions than to monovalent cations (By similarity). Acts as a calcium-activated calcium permeable channel which may operate as a endoplasmic reticulum (ER) Ca(2+)-leak channel, reducing the loading of the ER Ca(2+) store (PubMed:36520003). Regulates intracellular Ca2+ signals, ion channel activity, and cytokine release in the renal tissue (PubMed:36520003). Plays an important role in olfaction, amplifying cAMP-evoked cyclic nucleotide-gated (CNG) channel currents in the olfactory sensory neurons (By similarity). Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22178883). Can inhibit the activity of ANO1 (PubMed:20056604, PubMed:22946059).
Cellular Location	Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Note=Shows predominantly an intracellular localization with a weak expression in the cell membrane
Tissue Location	Expressed in the kidney (PubMed:36520003). Expressed in the olfactory epithelium (PubMed:36330924)