癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
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DTX2 Rabbit pAb
DTX2 Rabbit pAb
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- 背景知识
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q86UW9 |
| Predicted | Rat, Human, Mouse, Zebrafish, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 67246 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human DTX2 |
| Epitope Specificity | 331-430/622 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasmic and Nuclear. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | DTX2 belongs to the Deltex family. It contains one RING-type zinc finger and two WWE domains. DTX2 is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. It probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context; mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. DTX2 also functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases. There are two named isoforms. |
| Gene ID | 113878 |
|---|---|
| Other Names | Probable E3 ubiquitin-protein ligase DTX2, 2.3.2.27, DTX2, KIAA1528, RNF58 |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DTX2 |
|---|---|
| Synonyms | KIAA1528, RNF58 |
| Function | E3 ubiquitin ligase that conjugates ubiquitin to ADP- ribosylated cysteine residues, independently of lysine side chains or free N-termini (PubMed:40681873). For example, PARP7 generates an ADP- ribosyl degron within the DNA-binding domain of the androgen receptor (AR), which is recognized by DTX2 and targeted for proteasomal degradation (PubMed:40681873). Functions in genome stability maintenance by acting as an ADP-ribosylation-dependent regulator of DNA double-strand break repair (PubMed:38992439). Mechanistically, recognizes PARylated substrates generated in response to DNA damage and promotes their ubiquitination via its RING and DTC domains (PubMed:38992439). Also regulates additional cellular pathways through ubiquitination of specific substrates. Acts as an E3 ubiquitin ligase for RNA demethylase FTO, promoting its ubiquitination and proteasomal degradation (PubMed:39661064). In turn, regulates antitumor immune responses (PubMed:39661064). Promotes 'Lys-27'-linked ubiquitination of TFRC, thereby modulating iron metabolism and inhibiting ferroptosis (PubMed:41772062). In addition, promotes transcription of telomerase reverse transcriptase by mediating 'Lys-63'-linked ubiquitination of nuclear factor 1 C-type (NFIC) that stabilizes its binding on hTERT promoter (PubMed:35198878). |
| Cellular Location | Chromosome. Cytoplasm. Nucleus Note=Predominantly cytoplasmic. Partially nuclear. Is recruited to DSBs in a PARylation-dependent manner (PubMed:38992439) |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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