CAPRIN2 Rabbit pAb

Sample:
Lane 1: U251 (Human) Cell Lysate at 30 ug
Lane 2: U87MG (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-CAPRIN2 (AP54851) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120-140 kD
Observed band size: 140 kD
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (AP54851) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-CAPRIN2 antibody(AP54851), Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q6IMN6
Reactivity	Rat, Human
Predicted	Pig, Mouse, Chicken, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	125925 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human CAPRIN2
Epitope Specificity	101-200/1127
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm; Mitochondrion. Colocalizes with aggregated mitochondria and Mitochondrion. Cytoplasm. Expressed throughout the cytoplasm.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Caprin2 is 1127 amino acid highly conserved protein that is ubiquitously expressed with highest levels of expression in brain and spleen. Caprin2 stabilizes cytosolic β-catenin and enhances LEF-1 dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Caprin2 promotes LRP5/6 phosphorylation by GSK-3 and enhances the interaction between Axin and LRP5/6. It is suggested that Caprin2 functions as a proapoptotic inhibitor of the cell cycle. Nine isoforms of Caprin2 exist due to alternative splicing events.

Additional Information
Gene ID	65981
Other Names	Caprin-2, C1q domain-containing protein 1, Cytoplasmic activation/proliferation-associated protein 2, Gastric cancer multidrug resistance-associated protein, Protein EEG-1, RNA granule protein 140, CAPRIN2 {ECO:0000312\|EMBL:AAI17673.1}
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CAPRIN2 {ECO:0000312\|EMBL:AAI17673.1}
Function	Promotes phosphorylation of the Wnt coreceptor LRP6, leading to increased activity of the canonical Wnt signaling pathway (PubMed:18762581). Facilitates constitutive LRP6 phosphorylation by CDK14/CCNY during G2/M stage of the cell cycle, which may potentiate cells for Wnt signaling (PubMed:27821587). May regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons (By similarity). Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation (PubMed:14593112). May be involved in apoptosis (PubMed:14593112).
Cellular Location	[Isoform 1]: Cytoplasm. [Isoform 5]: Mitochondrion. Note=Colocalizes with aggregated mitochondria
Tissue Location	Detected in all tissues tested with highest levels of expression in brain and spleen.