癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Phospho1 Rabbit pAb
Phospho1 Rabbit pAb
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- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q8TCT1 |
| Reactivity | Human, Mouse |
| Predicted | Rat, Dog, Pig, Horse, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 29713 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Phospho1 |
| Epitope Specificity | 201-267/267 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily. |
| Gene ID | 162466 |
|---|---|
| Other Names | Phosphoethanolamine/phosphocholine phosphatase, 3.1.3.75, PHOSPHO1 {ECO:0000303|PubMed:12464021, ECO:0000312|HGNC:HGNC:16815} |
| Target/Specificity | Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | PHOSPHO1 {ECO:0000303|PubMed:12464021, ECO:0000312|HGNC:HGNC:16815} |
|---|---|
| Function | Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed:15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity). |
| Cellular Location | Extracellular vesicle {ECO:0000250|UniProtKB:Q8R2H9}. Note=Localizes to special class of extracellular vesicles, named matrix vesicles (MVs), which are released by osteogenic cells. {ECO:0000250|UniProtKB:Q8R2H9} |
| Tissue Location | Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily.
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