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PKNOX1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - PKNOX1 Polyclonal Antibody AP54938
    Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PKNOX1) Polyclonal Antibody, Unconjugated (AP54938) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF, ICC
Primary Accession P55347
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 47607 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human PKNOX1
Epitope Specificity 21-120/436
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Belongs to the TALE/MEIS homeobox family. Contains 1 homeobox DNA-binding domain.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Gene ID 5316
Other Names Homeobox protein PKNOX1, Homeobox protein PREP-1, PBX/knotted homeobox 1, PKNOX1, PREP1
Target/Specificity Ubiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow.
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name PKNOX1 {ECO:0000303|PubMed:9479508, ECO:0000312|HGNC:HGNC:9022}
Function Activates transcription in the presence of PBX1A and HOXA1.
Cellular Location Nucleus.
Tissue Location Ubiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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