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STCH/HSPA13 Rabbit pAb

STCH/HSPA13 Rabbit pAb

     
  • 1 - STCH/HSPA13 Rabbit pAb AP54991
    Sample: Hela Cell Lysate at 40 ug
    Primary: Anti-STCH/HSPA13 (AP54991) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 50 kD
    Observed band size: 50 kD
  • 14 - STCH/HSPA13 Rabbit pAb AP54991
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STCH) Polyclonal Antibody, Unconjugated (AP54991) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession P48723
Reactivity Human, Mouse
Predicted Rat, Dog, Pig, Horse, Rabbit, Sheep, Chimpanzee
Host Rabbit
Clonality Polyclonal
Calculated MW 51927 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human STCH/HSPA13
Epitope Specificity 41-140/471
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Microsome. Endoplasmic reticulum.
SIMILARITY Belongs to the heat shock protein 70 family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein. [provided by RefSeq, Jul 2008]
Additional Information
Gene ID 6782
Other Names Heat shock 70 kDa protein 13, Heat shock protein family A member 13, Microsomal stress-70 protein ATPase core, Stress-70 protein chaperone microsome-associated 60 kDa protein, HSPA13, STCH
Target/Specificity Constitutively expressed in all tissues.
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name HSPA13
Synonyms STCH
Function Has peptide-independent ATPase activity.
Cellular Location Microsome. Endoplasmic reticulum.
Tissue Location Constitutively expressed in all tissues.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein. [provided by RefSeq, Jul 2008]

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