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>   首页   >   产品   >   一抗   >   癌症   >   HHAT Antibody (N-term)   

HHAT Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HHAT Antibody (N-term) AP5503a
    HHAT Antibody (N-term) (Cat.#AP5503a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).
  • 14 - HHAT Antibody (N-term) AP5503a
    HHAT Antibody (N-term) (Cat. #AP5503a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - HHAT Antibody (N-term) AP5503a
    HHAT Antibody (N-term) (Cat. #AP5503a) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q5VTY9
Other Accession NP_001116306.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57313 Da
Antigen Region 30-59 aa
Additional Information
Gene ID 55733
Other Names Protein-cysteine N-palmitoyltransferase HHAT, 231-, Hedgehog acyltransferase, Melanoma antigen recognized by T-cells 2, MART-2, Skinny hedgehog protein 1, HHAT, MART2, SKI1
Target/Specificity This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHHAT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HHAT
Synonyms MART2, SKI1
Function Palmitoyl acyltransferase that catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling (PubMed:18534984, PubMed:24784881, PubMed:31875564). It also catalyzes N-terminal palmitoylation of DHH (PubMed:24784881). Promotes the transfer of palmitoyl-CoA from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane, where SHH palmitoylation occurs (PubMed:31875564). It is an essential factor for proper embryonic development and testicular organogenesis (PubMed:24784881).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Co-localizes with SHH in the ER and Golgi membrane.
Tissue Location Widely expressed. Expressed in fetal ovary and testis, with high levels of expression observed in Sertoli cells (PubMed:24784881).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).

REFERENCES

Chamoun, Z., et al. Science 293(5537):2080-2084(2001)
Kawakami, Y., et al. J. Immunol. 166(4):2871-2877(2001)

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